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Production of Polyhydroxybutyrate (phb) by Pseudomonas Putida Strain kt2440 on Cassava Hydrolysate Medium

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Author Affiliations

  • 1Department of Chemical Engineering, Ladoke Akintola University of Technology, Ogbomoso, NIGERIA
  • 2Department of Chemical Engineering, Obafemi Awolowo University, Ile-Ife, NIGERIA
  • 3National Biotechnology Development Agency (NABDA), Abuja, NIGERIA

Res.J.chem.sci., Volume 1, Issue (4), Pages 67-73, July,18 (2011)

Abstract

The potential of local strain of Pseudomonas putida strain KT2440 in polyhydroxbutyrate production was investigated in this study. This was done to establish the capabilities of local strains to utilize renewable and locally available substrates in polyhydroxybutyrate production. It involved hydrolysis of extracted starch from freshly harvested cassava tubers using enzyme-enzyme hydrolysis method, followed by aerobic fermentation using Pseudomonas putida in batch cultures on a mixture of the hydrolysate and nutrient media. The reducing sugar hydrolysate served as the carbon source while di- ammonium sulphate was the source of Nitrogen. The reaction temperature, pH and agitation rate in the fermentor were maintained at 30 C, 7.5 and 400 rpm respectively. The biomass growth was measured by cell dry weight and the polyhydroxybutyrate content measured by gas chromatographic method. The results obtained showed that the medium supported the growth of the organism. After 72 h fermentation, the substrate consumption by the organism was 8.88 g/l to give a dry cell weight of 0.91 g/l, resulting in a biomass yield on substrate (Yx/s) of 0.1025 g g-1. The gas chromatographic analysis gave a final polyhydroxybutyrate value of 0.2285 g/l with corresponding product yield on biomass (Y p/x) of 0.2511 g g-1 (25.11%) and product yield on substrate (Yp/s) of 0.0257 g g-1. It can be concluded that Pseudomonas putida strain KT2440 has capability to utilize cheap, renewable and locally available substrates in polyhydroxybutyrate production. Also, comparing the result of the present study with those from the previous ones showed that although Pseudomonas putida strain KT2440 accumulated polyhydroxybutyrate lower than the usual value in excess of 50 % of the cell dry weight given by various strains of microorganisms during polyhydroxybutyrate production it is a potential candidate for polyhydroxybutyrate production.

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